Yesterday's question-what are the various types of products that need microbial testing and what are the limits for the various bacteria and yeast and mold?
Answer-Washington State has three categories of products that require microbial testing unprocessed material (buds, flower,etc. shorthand- upm), processed material (keif, shorthand- pm) and solvent-based extracts (bho,rso,pho, shorthand-sbe). I will list the allowed limits for each below
Total aerobic plate count upm-100,000
Yeast and mold
Bile tolerant gram-negative bacteria
e-coli and salmonella are not allowed to be present in any samples, if these are detected in any amount, the whole lot must be destroyed. In addition to all of this there are retest limits, the retest limit is a multiple of the maximum allowance of cfu per gram (the numbers listed above) with total aerobic count being two times the upper limit yeast and mold being five times the upper limit coliforms being two times the upper limit and bile tolerant gram-negative being two times the upper limit.
Microbial testing requires meticulous sterile technique which is the hardest part imo. The cfu/g is calculated by counting the colonies on the plates and multiplying by the dilution factor (ya know, math). In today's picture we have a bunch of counted plates ready for disposal. So if we determine all the cfu/g by counting the plates, how do we determine if salmonella and ecoli are present?... find out tomorrow!